丹参、赤芍、莪术对小鼠Lewis肺癌血管生成的影响

Influence of Dan-shen,Chi-shao and E-zhu on the Tumor Angiogenesis of Mice with Lewis Lung Cancer

目的:探讨中药丹参、赤芍、莪术对肿瘤血管生成的影响。方法:将上述3味中药分别水煎浓缩后,分别进行动物实验,实验的方法一致。将Lewis肺癌模型小鼠随机分为生理盐水对照组及中药低、中、高剂量组(丹参剂量分别为12、16、20g/kg,赤芍及莪术剂量均分别为6、8、10g/kg),每组小鼠10只,每天灌胃1次,连续20d。第22天处死小鼠,免疫组织化学计数肿瘤组织微血管密度(MVD)及检测血管内皮细胞生长因子(VEGF)的表达。结果:中高剂量丹参、莪术及高剂量赤芍能提高肿瘤组织MVD及VEGF表达,P<0.05或P<0.01。结论:单味中药煎剂丹参、赤芍、莪术会促进小鼠Lewis肺癌血管生成。

Objective ：To explore the affecting effects of danshen,chishao and ezhu on mice with Lewis Lung Cancer （LLC）. Methods： LLC cells were inoculated subcutaneously into C57BL mice .The three kinds of Chinese herb were experimented in the same way, respectly ,Forty mice were randomly divided into normal saline group （control group ,n-10）and experimental groups （danshen of 12,16and 20g·kg^-1,10 in each group, chishao and ezhu of 6,8 and 10 g·kg^-1,respectively ,10 in each group ）. On 22th day ,the mice were killed. Tumor microvessel density （MVD） was quantitated by immunohistochemistry using monoclonai antibodies against factor FS, vascular endothelial growth factor （VEGF） immunoreactivity was examined by immunohistochemistry using polyclonal antibodies against VEGF. Results： The MVD and VEGF on middle ,high dosages ofdanshen and ezhu groups,and on high dosage of chishao group were significantly higher than control group, P 〈0.05 or P 〈0.01 o Conclusion： Danshen,chishao and eshu can enhance tumor angiogenesis of the implanted LLC in C57BL mouse.