摘要
复杂类型的脲氮吸附物,在明胶膜(IE ) 由 chitosancoated 二醛纤维素(CDAC ) 和使不能调动的 urease 组成,被准备。纤维素,二醛纤维素(数模转换器) 和 CDAC 被扫描电子显微镜描绘。结果显示纤维素 C2-C3 契约在氧化 ofperiodate 下面被打破,它是到数模转换器的 oxidated。数模转换器与去乙酰壳多醣是涂的, CDAC 被获得。脲氮的吸附在间歇式系统被学习到在 Na_2HPO_4-NaH_2PO_4 缓冲液的吸附物上。实验参数的效果包括氧化 ofCDAC,起始的脲氮集中,酸碱值和温度的度,在脲氮的吸附能力上到在 CDAC/IE 重量比率 10 点的吸附物上:1 被调查。结果显示这些参数显著地影响了吸附能力。脲氮的吸附能力是在 CDAC 88 百分比的氧化的度的 36.7 mg/g 到吸附物上,起始的脲氮集中 600 mg/L,酸碱值 7.4 和温度 37 deg C。
The urea nitrogen adsorbent of complex type, which consists of chitosan coated dialdehyde cellulose (CDAC) and immobilized urease in gelatin membrane (IE), was prepared. The cellulose, the dialdehyde cellulose (DAC) and the CDAC were characterized by scanning electronic microscope. The results indicate that the cellulose C2-C3 bond was broken under the oxidation of periodate and it was oxidated to DAC. The DAC was coated with chitosan and the CDAC was obtained. The adsorption of urea nitrogen onto the adsorbent in Na2HPO4-NaH2PO4 buffer solution was studied in batch system. The effects of the experiment parameters, including degree of oxidation of CDAC, initial urea nitrogen concentration, pH and temperature, on the adsorption capacity of urea nitrogen onto the adsorbent at CDAC/IE weight ratio 10:1 were investigated. The results indicate that these parameters affected significantly the adsorption capacity. The adsorption capacity of urea nitrogen onto the adsorbent was 36.7 mg/g at the degree of oxidation of CDAC 88%, initial urea nitrogen concentration 600 mg/L, pH 7.4 and temperature 37℃.
基金
SUPPORTED BY NATURAL SCIENCE FOUNDATION OF TIANJIN ( NO. 033802011).
关键词
复型吸附剂
脲氮
吸附能力
生物催化作用
壳聚糖
二醛基纤维素
固氮
adsorption
urea nitrogen
complex type adsorbent
chitosan coated dialdehyde cellulose
immobilized urease
bio-catalysis