黑米皮花色苷对动脉硬化斑块稳定性影响

Effect of anthocyanin-rich extract from black rice on stability of atherosclerotic vulnerable plaque

目的探讨黑米皮花色苷提取物对载脂蛋白E基因缺陷(ApoE-/-)小鼠动脉粥样硬化(As)斑块稳定性及血脂的影响。方法48只ApoE-/-小鼠(C57BL/6J品系)饲喂美国营养学会1993年版啮齿类动物纯化饲料(AIN-93)30周,建立As不稳定斑块动物模型,随机分为黑米皮花色苷组、辛伐他汀组及对照组。膳食干预20周后观察无名动脉斑块形态学变化,测定不稳定斑块出现频率、相对面积和血脂水平。结果黑米皮花色苷组和辛伐他汀组与对照组相比,血清TG、TC、LDL-C、HDL-C含量显著降低,LDL-C/HDL-C比值差异无统计学意义(P>0.05),不稳定斑块出现频率减少,斑块相对面积分别降低15.77%与14.72%(P<0.05),但3组间血清总抗氧化能力差异无统计学意义(P>0.05)。结论黑米皮花色苷提取物能促进ApoE-/-小鼠晚期斑块的稳定性,其作用效果类似于他汀类药物,该作用可能与其改善脂代谢有关,而与抗氧化能力无关。

Objective To explore the role of anthocyanin-fich extract from black rice on anti-atherosclerosis. Methods A total of 48 ApoE^-/- mice on a C57BL/6J back ground were fed with a chow diet based on American Institute of Nutrition-93 purified diets for laboratory rodents（AIN-93）for 30 weeks to establish vulnerable plaque model. Then the mice were randomly divided into three groups： anthocyanin-rich extract from black rice group, simvastatin group and control group. The animals were fed different diets for 20 weeks, then the changes of vulnerable plaque in innominate arteries were observed and the blood was determined for lipids. Results Compared with control group, levels of serum TG, total cholesteral（TC）, low-density lipoprotein cholesteral（LDL-C）, high-density lipoprotein cholesteral（HDL-C） were all significantly decreased in anthocyanin-rich extract from black rice group and simvastatin group （P〈0.05）. And there is no difference in LDL-C/HDL C a mong three groups. The frequency of vulnerable plaque in innominate arteries was lower and the relative plaque area was smaller, decreased by 15.77 % and 14.72% separately, in anthocyanin-rich extract from black rice group and simvastatin group than in control group. But there was no significant difference in total anti-oxidant capacity（T-AOC）among three groups （P 〉 0.05 ）. Conclusion Similar to simvastain, antbocyanin-rich extract from black rice can promote ：he stability＇ of ad vanced plaque in old ApoE^-/- mice, which may contribute to modulate lipid metabolism, not the anti-oxidate capability.