内皮型一氧化氮合酶基因与年龄、吸烟的交互作用

Interaction between endothelial nitric oxide synthase gene mutation and age,smoking in coronary heart disease

目的探讨内皮型一氧化氮合酶(eNOS)基因第7外显子G 894 T(Glu 298 Asp)变异与年龄、吸烟之间交互作用在冠心病患病中的意义。方法用病例—病例研究设计,以新诊断的冠心病患者为研究对象,用聚合酶链反应—限制性片段长度多态性分析法(PCR-RFLP)检测eNOS基因G 894 T变异,运用单因素及多因素Logistic回归分析eNOS基因G 894 T变异与年龄、吸烟之间的交互作用。结果eNOS基因G 894 T变异与年龄之间在冠心病的患病中存在显著的相乘模型交互作用,且该交互作用具有显著的剂量-反应关系;eNOS基因G 894 T变异与吸烟之间在冠心病的患病中无显著的相乘模型交互作用,其交互作用也无显著的剂量-反应关系。结论eNOS基因G 894 T变异可增加低年龄者冠心病的患病的患病危险性;且年龄越低,eNOS基因G 894 T变异对冠心病的患病危险性越高。

Objective To investigate the effect of interacion between endothelial nitric oxide synthase（eNOS）gene G 894 T mutation and age, smoking on the risk of coronary heart disease（CHD）. Methods Caseonly design was used. Newly diagnosed CHD patients were recruited as subjects and polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）was performed to detect eNOS gene G 894 T mutation. Interaction between eNOS gene G 894 T mutation and age, smoking was evaluated by odds ratios from simple and multiple logistic regression analysis. Results There was significant negative multiplicative model interaction between eNOS gene G 894 T mutation and smoking, nor was the dose-response relationship significant. Conclusion eNOS gene G 894 T mutation can lead to increased risk of CHD in younger groups, and the younger, the higher is the risk.