摘要
目的研究激活素A对体外培养的大鼠肾间质成纤维细胞增殖及激活的影响。方法取SD大鼠肾间质成纤维细胞进行体外培养,分别向培养液中加入激活素A或激活素A加卵泡抑素或卵泡抑素,应用MTT法检测细胞增殖情况(OD值),免疫组化及RT-PCR法检测培养细胞α-肌动球蛋白(-αSMA)的表达,以观察成纤维细胞的激活状况。结果与对照组和卵泡抑素组相比,激活素组成纤维细胞的OD值增高有统计学意义(P<0.05),表达-αSMA mRNA和蛋白显著增多。结论激活素A能促进肾间质成纤维细胞增殖和激活,提示激活素A可能在肾间质损害的发生发展中起重要作用。
Objective To study the stimulating effect of activin A on fibroblasts of rat kindney, Methods Fibroblasts separated from SD rat kindney were cultured in diferent medium contained aetivin A or actlvin A and follistatin(FS) or FS in different concentration, The proliferation of renal fibroblasts was assayed by MTT method. The α-SMA mRNA expression in cultured renal fibro blasts was examined by RT-PCR, The α-SMA protein expression in culutred renal fibroblasts was observed with immunocytochem istry. Results The rat renal fibroblasts stimulated with activin A had significant higher OD value and higher expression of α- SMAmRNA and protein than that of control group and group stimulated with FS, Conclusion The activin A could stimulate fibroblasts proliferating and activating, and might play an important role in the mechanism of renal fibrosis.
出处
《重庆医学》
CAS
CSCD
2006年第2期131-133,136,共4页
Chongqing medicine