摘要
本研究制备了抗18kDa粘连蛋白的单克隆抗体,并证实在不同血清型之间,甚至不同种衣原体之间18kDa粘连蛋白具有一定的共同抗原表位。我们建立的空斑形成试验和单克隆抗体空斑减少中和试验,实验结果明显、客观,易于判定,稳定性好。用此筛选出县有中和活性的2B9McAb。该McAb不同于目前已报道的McAb,其中和活性不依赖于补体,而可能是抑制衣原体的粘附。
In this study we have produced three McAbs against binding protien of chlamydia trachomatis. All the three McAbs could not only react with LGV2 serovar, but also with LGV1,D and I serovars and TWAR strain,suggesting that 18 kDa proteins of different serovars have common epitops. Using plaque technique we have established that LGV 2 cellular infection model which makes the evaluation of the LGV2 infection,is objective, stable and simple. A plaque inhibition test was performd using McAbs, and the results showed that there was neutralization activity with 2B9, but not with 1A8 and 1D9. Furthermore,unlike McAbs described by others,2B9 showed neutralization activity when complements was absent,suggesting that its activity was probably due to inhibitin of chlamydia adherance to host cells by blocking sites,but not due to complements mediated direct lysis of chlamydia. This study might provided us a new approach to the prevention and treatment of chlamydial diseases.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
1996年第3期49-52,共4页
Chinese Journal of Cellular and Molecular Immunology
关键词
衣原体
粘连蛋白
单克隆抗体
中和活性
空斑形成
chlamydia
binding protein
monoclonal antibody
neutralization
plaque test