流式细胞术测肺癌患者外周血T细胞亚群及其与临床病理的关系

T lymphocyte subsets in the peripheral blood of patients with lung carcinoma

目的:探讨应用流式细胞术检测肺癌患者细胞免疫功能的方法及其与临床病理的关系。方法:应用三色免疫荧光流式细胞仪测定105例肺癌患者及18例正常对照者外周血T细胞亚群CD3+、CD4+、CD8+、NK细胞、CD4+/CD8+比值。结果:全组肺癌患者外周血中各T淋巴细胞亚群比例较正常对照组无显著性差异;Ⅰ期、Ⅱ期肺癌患者和健康对照组之间T细胞各亚群相比没有统计学差异;Ⅲ期、Ⅳ期肺癌患者和健康对照组相比:CD4+明显降低(P=0.04);CD8+则明显升高(P=0.02);CD4+/CD8+较正常有所下降,但没有显著性差异。结论:外周血T细胞亚群的检测有助于对肺癌患者的病情、临床分期以及机体免疫状态的判断,晚期肺癌患者机体免疫状态成紊乱型改变。

Objective To observe the state of cellular immunity of patients with lung carcinoma and the relationship between clinical pathological outcome and T lymphocyte subproportion. Methods Venous blood was obtained from 105 patients with lung carcinoma and 18 healthy volunteers. Percentages of T lymphocyte subsets were measured by flow cytometry. Results Compared to controls, 105 patients with lung carcinoma had no significant differences in the percentages of CD3^＋, CD4^＋,CD8^＋ T cells and the CD4^＋/CD8^＋ ratio. Although there was no difference in the percentages of CD3^＋, CD4^＋and CD8^＋ T cells between the stage Ⅰ and Ⅱ and the controls, However, the patients with advanced carcinoma（stage Ⅲ, Ⅳ,） had significant higher percentages of CD8^＋T cells and lower CD4^＋T cells than that of the controls. Conclusion Analysis of T lymphocyte subset can provide useful information on the function of the patients with lung carcinoma.