山莨菪碱对肺损伤肺α_1受体及磷脂酶A_2的影响

Effects of anisodamine on pulmonary α_1-adrenergic receptor and phospholipase A_2 in acute lung injury

用放射性配基结合分析法，检测大鼠内毒素性肺损伤中肺α１肾上腺素受体（α１受体）的变化，并观察山莨菪碱对肺α１受体和磷脂酶Ａ２（ＰＬＡ２）的影响。结果表明，（１）ｉｖ内毒素（ＥＴ）后４ｈ，大鼠肺α１受体的最大结合容量明显降低，较对照组减少３４％，同时肺组织ＰＬＡ２激活及膜磷脂含量减少。（２）山莨菪碱能减轻ＥＴ诱导的大鼠急性肺损伤，其作用机制与阻滞α１受体，抑制ＰＬＡ２、防止膜磷脂降解、减少花生四烯酸的释放等有关。

Change of α1-adrenergic receptor(α1AR) in lung tissues during endotoxin-induced rat acute lung injury was measured with radioligand binding assay.The effects of anisodamine on pulmonary α1-AR and phospholipase A2(PLA2) were observed.The results showed that:(1) 4 hours after the endotoxin injection,there was a significant decrease in the maximal binding capacity of α1-AR by 34% as compared with the control group,meanwhile elevated activity of PLA2 in rat lung and reduction of the phospholipids content of cell membrane was found.(2)Anisodamine could attenuate endotoxin induced acute lung injury in rats.This effect might be related to anisodamine's blockage of α1-AR and suppression of PLA2,prevention of membranous phospholipids from degradation,and the reduction of arachidonic acid release.