摘要
采用高效液相色谱法(HPLC)法对5种用1-苯基2-甲基-5-吡唑啉酮(PMP)衍生后的单糖,进行了分离分析,并采用该方法对两种真菌源寡聚糖的单糖组成进行了分析。同时初步研究了真菌源寡聚糖的诱抗活性。结果表明两种真菌源寡聚糖均有诱抗活性,葡萄灰霉寡聚糖(寡聚糖Ⅱ)的诱抗活性高于葡萄链格孢寡聚糖(寡聚糖Ⅰ)的诱抗活性;用高效液相色谱法(HPLC)对单糖衍生物实现了良好的分离,且该方法可作为实际样品中痕量单糖分析的常规方法;两种寡聚糖中均含有果糖,D-甘露糖,葡萄糖,D-半乳糖和D-阿拉伯糖五种单糖,寡聚糖Ⅰ中该5种单糖的摩尔比为3:5:10:4:1,寡聚糖Ⅱ中该5种单糖的摩尔比为1:5:3:2:2。
A method for the derivatization of carbohydrates with 1-phenyl-3-methyl-5-pyrazolone （PMP） has been used and simplified. Five monosaceharide derivatives were well separated by high performance liquid chromatography （HPLC）. Furthermore, the developed method has been successfully applied for the analysis of monosaccharides in the oligosaccharides derived from cell wall of A lternaria alternate （oligosaccharides Ⅰ ） and Botrytis Cinerea （oligosaccharides Ⅱ ）.HPLC analysis showed that the oligosaccharides consisted of fructose, D-mannose,ghicose, D-galactose and D - arabinose. The proportion of the five monosaccharides in oligosaccharides Ⅰ was 3：5：10：4：1 and the proportion of the five monosaccharides in oligosaccharides Ⅱ was 1：5：3：2：2 The biological activity of the oligosaccharides Ⅱ was much higher than the oligosaccharides Ⅰ.
出处
《食品研究与开发》
CAS
北大核心
2006年第1期53-56,共4页
Food Research and Development
基金
天津市自然基金项目(033608711)
关键词
高效液相色谱
寡聚糖
水解
单糖
衍生
high performance liquid chromatography
oligosaccharides
hydrolyze
monosaccharide
derivatization
