薄层层析-分光光度法测定通络解毒搽剂中盐酸小檗碱含量

Determination of berberine hydrochloride in Tongluo Jiedu liniment with Thin Layer Chromatography-Ultraviolet Spectrophotometry

目的:用薄层层析-分光光度法测定通络解毒搽剂中原料药材黄连的盐酸小檗碱含量。方法:用硅胶G薄层板点样,以正丁醇-冰乙酸-水(14∶2∶4)展开,于365nm波长定位分离,测定波长为344nm。结果:盐酸小檗碱在1.02￣5.10μg/ml范围内浓度C与吸光度A呈良好的线性关系(r=0.9999),加样回收率为(98.78±2.64)%,RSD为2.67%。结论:本法操作简便,结果可靠,可作为通络解毒搽剂的质量控制方法。

Objective： A determination of amount of berberine hydrochloride in Rhizoma Coptidis and Tonghto Jiedu liniment with Thin Layer Cheetrophotometry-Ultraviolet Spectrophotometry was established. Methods： The thin layer plate was Silica Gel-3% CMC-Na （0.4 mm）, The developer was Butanol Acetic Acid-Water（14：2：4）, The characteristic spot was distinguished and separated at 365 nm. UV deteetion wavelength was 344 nm. Results： The correlation of the linear range of berbenne hydroehloride was good in 1.02 - 5.10 μg/ml （r = 0.9999）, The average recovery was （98.78±2.64）% （RSD = 2.67%）. Conclusions： The method is simple, highly sensitive and reproducible. It may be used for the quantitative determination of the liniment.