肾上腺髓质素对大鼠局灶性脑缺血再灌注后脑组织的保护作用(英文)

Protection of adrenomedullin after focal cerebral ischemia/reperfusion in rats

目的探讨肾上腺髓质素与脑缺血再灌注损伤的关系。方法采用栓线法制成大鼠大脑中动脉缺血再灌注模型, 阻断血流 2 h 后进行再灌注。应用免疫组织化学染色法检测不同时间段大鼠局灶性脑缺血再灌注后肾上腺髓质素的表达情况, 并进行动态观察。结果正常大鼠脑内即有肾上腺髓质表达, 假手术后肾上腺髓质素表达略有增加, 但与正常对照组相比无明显差异, P >0.05; 大鼠脑缺血再灌注后肾上腺髓质素免疫阳性细胞增多, 与正常对照组及假手术组相比差异显著, 均 P <0.05; 大鼠脑缺血再灌注后缺血侧既缺血对侧肾上腺髓质素免疫阳性细胞均增多, 但以缺血侧区域增多最为明显, P <0.05。动态观察发现, 脑缺血再灌注 2 h 后, 肾上腺髓质素免疫阳性细胞即增多, 缺血再灌注 6 h 达高峰, 至 1 周左右仍明显增多。结论脑缺血再灌注后肾上腺髓质表达增强。

【Objective】 To investigate the effect of Adrenomedullin （ADM） after middle cerebral artery occlusion （MCAO） in rats. 【Method】 Using the model of middle cerebral artery occlusion （MCAO） in rats, 2 hours isehemia followed by various time of reperfusion, expression of ADM at the each time point by immunohistochemistry, continuously. 【Results】 ADM expressed in normal rats, and increased a little in sham group, without significant difference compared to the control group （P〉0.05）; after cerebral ischemia/reperfusion, ADM positive cells increased in ischemia cortex, with significant different compared to the normal control group and the sham group （P〈0.05）; ADM positive cells increased in ischemia sector and contralateral after cerebral ischemia reperfusion, but it was more evidence in ischemia area （P〈0.05）. Observing continuously, the number of ADM positive cells increased after reperfusion of 4 hours post-MCAO, reaching climax after 6 hours post-MCAO, and remained elevated for up to 7 days. 【Conclcsions】 The expression of ADM increased for its cerebrovascular function after cerebral ischemia/reperfusion.