P-选择素在吸烟大鼠脑血管内皮细胞的表达

Expression of P-selectin on vascular epithelium in smoked rat brain

目的观察不同吸烟量的大鼠脑内血管内皮细胞P-选择素的表达,初步探讨吸烟导致脑缺血的发病机制。方法健康Wistar大鼠60只,分为正常对照组,短期大量吸烟组,长期大量吸烟组,长期小量吸烟组,戒烟组及非吸烟组。通过免疫组化法观察大鼠脑血管内皮细胞P-选择素的表达。结果正常对照组及非吸烟组P-选择素表达极少。余各组可见有不同程度表达。且与对照组比较差异有显著性(P<0.05)。结论吸烟可致脑内血管内皮细胞上P-选择素表达增加,可能是吸烟致脑梗死的主要途径之一。

【Objective】 Observing expression of P-selectin on vascular epithelium in smoked rat brain to discuss the cause that smoking cause ischemia in brain. 【Methods】 60 healthy Wistar rats were divided into 6 groups, norreal group, short-time and big-dose smoking group, long-time and big-dose smoking group, long-time and small-dose smoking group, giving-up smoking group, control group. We obtained brain following experiment ending and observed expression of P-seleetin in smoked rat by immunohistochemistry stain. 【Results】 The expression of P-selectin in normal group and control group is weak. The significant different expression is observed in other groups （P 〈 0.05）. Giving up smoking can reduce the expression of P-selectin. 【Conclusions】 Smoking can make P-selectin express more in endothelial cell. There is prominent dose-depend and time-depend relationship between smoking and expression of P-selectin. Giving up smoking can reduce expression of P-selectin. The increase of P-seleetin plays an important role in ICVD caused by smoking.