L-乳酸菌的选育及在酒糟基质中的培养

Screening of L-lactic acid-producing Strain and Its Prime Culture in the Substrate of Distiller's Grainst

从市售酸乳、泡菜中筛选得到产L-乳酸细菌,通过NTG诱变选育和摇瓶发酵试验,选出两株利用葡萄糖较好的菌株m-58和m-16,以玉米浓醪酒糟清液为基质对2株乳酸细菌进一步驯化培养,得出m-58菌株,更能较好地发酵玉米酒糟清液,产生乳酸,m-58菌株在48h发酵含糖63g/L的玉米浓醪酒糟清液产生50g/L的L-乳酸,m-58菌株适合用于发酵玉米酒糟产乳酸。(孙悟)

L-lactic acid-producing bacteria were screened from sour milk and marinated vegetables, and after times of NTG mutation screening and flask shaking fermentation tests, two strains including m-58 strain and m-16 strain were obtained, then after further domesticated cultivation of both the two strains in the clear solution of distiller＇s grains of very high gravity （VHG） ethanol fermentation from corn, m-58 strain was thought to be a good strain for its efficient utilization of the substrate of VHG ethanol fermentation from corn ,which could produce 50 g/L L-lactic acid by fermenting 63 g/L corn high gravity mash distiller＇s grains within 48 h.