检测血管紧张素原基因M235T多态性的两种方法比较

Comparison of two methods to detect the angiotensiongen gene M235T polymorphism

目的对用于检测血管紧张素原基因M235T多态性的突变基因分离聚合酶链反应法(MS-PCR)和聚合酶链反应-限制性片段长度多态性分析法(PCR-RFLP)进行比较。方法分别用MS-PCR和PCR-RFLP检测82例住院患者的基因型及等位基因频率,并进行比较。并将用这两种方法测得的T等位基因发生频率与当地人群的总体发生率分别进行比较。结果两种方法所测得的AGT基因型分布及等位基因发生频率差异均有统计学意义(P<0.01),T等位基因发生率分别为0.87、0.71,后者更符合亚洲及我国人群(0.63～0.79;0.63～0.82)。结论在建立了最佳实验体系的前提下,选择传统的PCR-RFLP结果更加可靠。

Objetive To compare the mutagenically separated allele-specific polymerase chain reaction technique （MS-PCR）and polymerase chain reaction-restriction fragment length polymorphism methods （PCR-RFLP） used to detect the angiotensinogen gene M235T polymorphism. Methods MS-PCR and PCR-RFLP were used to detect the distribution of genotype and allele frequency of angiotensinogen gene in 82 patients, and the gene polymorphism results by two different methods were compared. Also, the T allele frequency detected by two methods would be compared with the total frequency in local population. Results Compared with the results （T allele frequency 0.87） detected by MS-PCR, the distribution of genotype and allele frequency （T allele frequency 0.71 ） detected by PCRRFLP showed significant difference （P〈O.O1）. The allele frequency detected by PCR-RFLP was accord with that of population in Asia and our country （0.63-0.79; 0.63-0.82）.Conclusion Traditional PCR-RLFP used to detect angiotensiongen gene polymorphism is more reliable.