AT1R基因A1166C多态性与原发性高血压血脂水平及左室重构的关系

The relationship between polymorphism of angiotensin Ⅱ type 1 receptor gene and essential hypertension

目的探索血管紧张素Ⅱ1型受体(AT1R)基因A1166C多态性与原发性高血压(EH)的相关关系及其对血脂水平和左室重构的影响。方法328例EH患者,571例健康人为正常对照。多聚酶链式反应(PCR)-限制性酶切法检测AT1R基因1166位点基因型。结果男性EH组AC基因型频率显著高于对照组(0.239vs0.086,P=0.001),OR=3.49,95%CI(1.226～5.899);女性EH组AC基因型及C等位基因频率均高于对照组,但差异无统计学意义。高血压危险因素的非条件Logistic回归分析表明,年龄、性别、有高血压家族史是EH的独立危险因素,基因型未进入模型。多元线性回归分析,SBP与LVMI成线性相关。结论AT1R基因AC基因型及C等位基因参与了男性原发性高血压的发病,但其作用是微效的。AT1R基因可能不是通过对血脂的影响参与EH发病的。AT1RA1166C基因多态性可能与高血压左室重构无关。

Objective To investigate the relationship between polymorphism of angiotensin Ⅱ type 1 receptor （AT1R） gene and essential hypertension （EH）, and the association between the AT1R gene polymorphism and plasm lipid level and LVMI （left ventricular mass index） were also studied. Methods 328 EH patients are collected as EH group, with 571 healthy people as control group. Polymerase chain reaction combined with restriction enzyme digestion was used to determine the polymorphism of the 1166 site of AT1R gene. Results The frequency of AC genotype in male EH group was higher than that in control group （0.239 vs 0.086, P〈0.001）, with OR value 3.49 （95% CI=1.226- 5.899）. The frequencies of AC genotype and C1166 allele of AT1R gene in female EH patients were also higher than that in control group, but there was no statistical significance. Logistic regression analysis showed that age, gender, and family history of EH were independent risk factors of EH. Polymorphism of the gene failed to enter the model. There was linear relation between SBP and LVMI. Conclusion Al166C polymorphism of AT1R gene was related with EH and Cl166 allele of AT1R gene may play a role in essential hypertension in males. However, it is not an independent risk factor for EH. There was no association between AT1R gene polymorphism and LVMI. The contribution of AT1R gene to the development of EH was independent of plasma lipid levels.