冠心病患者血清可溶性Fas和TNF-α水平变化

The change of serum concentration of soluble Fas and TNF-α in patients with coronary heart disease

目的探讨血清可溶性Fas(sFas)和TNF-α水平与冠心病(CHD)之间的关系。方法采用酶联免疫吸附双抗体夹心ABC-ELISA方法测定57例CHD患者(CHD组)和23例对照组受试者血清sFas和TNF-α水平。结果CHD患者血清sFas水平高于对照组(P<0.01),TNF-α水平也高于对照组(P<0.05),且CHD患者血清sFas水平与TNF-α成正相关(r=0.289,P=0.029)。CHD患者中sFas水平不稳定型心绞痛(UA组)患者和急性心肌梗死(AMI组)患者高于稳定型心绞痛(SA组)患者(P<0.05)。结论高水平的血清sFas和TNF-α与CHD有关,可能通过细胞凋亡和炎症反应途径参与冠状动脉粥样硬化斑块的发生发展。

Objective To investigate the relationship between serum levels of soluble Fas （sFas） ,tumor necrosis factor-α （TNF-α）and coronary heart disease （CHD）. Methods Enzyme-linked immunosorbent assay （ELISA） was used to measure the levels of sFas and TNF-α in the sera from 57 patients with CHD and 23 subjects without CHD as controls . Results Significant increases in the concentrations of sFas and TNF-α（ P〈0.01 and P〈0.05 , respectively） were found in the serum from patients with CHD compared to the control group. Moreover, sFas and TNF-α were positively correlated in patients with CHD （r= 0.289,P=0.029 ）. The serum level of sFas in patients with AMI or UA were higher than that in patients with SA （P〈0.05）. Conclusion High levels of sFas and TNF-α have relationship with CHD. They may be contribute to the development of atherosclerotic plaque by cell apoptosis and inflammatory response.