小红参含药血清对链球菌抗原刺激小鼠的免疫药理学研究

Serum Immunopharmacological Assessment of Effects of Rubia Yunnanensis on Murine Immune Cell Stimulated with Streptococcal Antigens

目的利用血清药理学实验方法,评价小红参含药血清对链球菌抗原刺激小鼠的免疫药理学效应,初步探讨小红参治疗银屑病的作用机制。方法利用β溶血型链球菌的超声破碎抗原为刺激物,观察小红参含药血清对链球菌致敏小鼠脾脏淋巴细胞的增殖活性,以及对小鼠腹腔巨噬细胞分泌一氧化氮(NO)的影响。结果醇提物各剂量组以及水提物高剂量组与空白血清组比较有显著性差异,均对链球菌抗原致敏小鼠的T淋巴细胞增殖具有抑制作用。醇提物高、中剂量组以及水提物高剂量组与空白血清组比较有显著性差异,均能抑制链球菌抗原致敏小鼠的腹腔巨噬细胞NO的分泌水平。结论小红参含药血清能够明显抑制T淋巴细胞对链球菌抗原的增殖作用;小红参含药血清可以抑制链球菌抗原诱导的小鼠腹腔巨噬细胞NO的分泌。

Serum pharmacological assessment： the effect of Rubia yunnanensis extract on the immune function stimulated by superantigens of streptococcus. Methods Using cell culture technique, mouse spleen lymphocytes and peritoneal macrophages were culture with superantigens of streptococcus and serum of Rubia yunnanensis. Lymphocytes proliferation stimulated by superantigens of streptococcus measured by MTT assay. NO level in mouse peritoneal macrophages affected by Rubia yunnanensis at different concentrations were determined and compared. Results Serum of Rubia yunnanensis significantly inhibit the proliferation of lymphocytes stimulated by superantigens of streptococcus, compared with normal control. NO content of macrophages culture in Rubia yunnanensis extract group were lower than those of control. In these tests, the effect of the extract by alcohol is more obvious than that of extract by water. Conclusions Rubia yunnanensis extract has effect in inhibiting immune function and anti-infect activity, it could be applied in clinical practice for immunoregulation and treatment of psoriasis.