HPLC法测定妇科十味胶囊中阿魏酸的含量

Determination of Ferulic Acid in Fuke Shiwei Jiaonang Capsule by HPLC

目的建立HPLC测定阿魏酸含量的方法。方法以反相高效液相色谱法测定阿魏酸的含量。色谱柱:HanbonLichrospher(250mm×4.6mm,5μm);流动相:乙腈-0.085%磷酸溶液(17∶83);检测波长:312nm。结果阿魏酸在0.0204～0.102μg浓度范围内线性关系良好(r=0.9999,n=5);平均加样回收率为98.32%,RSD=1.64%。结论该方法快速、简便、准确,重复性好。

Objective To develop the method for determining the concert of Ferulic acid. Method The Ferulic acid in Fuke Shiwei Jiaonang capsule was determinined by HPLC. The analytical column was Hanbon Lichrospher （250 mm × 4.6 mm × 5 μm） and mobile phase was Acetonitrile-0.085% phosphate acid（17 ： 83）. Ferulic acid was detected at 230 nm. Results In the scope of 0.0204-0.102 μg, the contents of Ferulic acid had good liner relationship with their peak area （r=0.9999, n=5）. The average recovery rate was 98.32%, RSD was 1.64% （n=5）. Conclusion The method was rapid, simple and precise.