茶多酚对肺癌SPC-A1细胞体外增殖及细胞周期的影响

The effects of tea polyphenols on proliferation and cell cycle of cell line SPC-A1 in lung cancer

目的探讨茶多酚对人肺癌SPC-A1细胞增殖和细胞周期的影响。方法体外培养人肺腺癌SPC-A1细胞，以50～300mg／L茶多酚作用12小时、24小时和36小时后，用MTr法测定细胞的增殖活性，透射电镜检测细胞凋亡情况，流式细胞仪测定细胞周期的阻滞作用。结果MTT法显示茶多酚可抑制肺癌细胞的增殖活性，其抑制作用随时间的延长而增强，以36小时抑制作用最强。在浓度50—150mg／L范围内，抑制作用随浓度增高而增强；当浓度大于150mg／L时，抑制作用随浓度增高反而减弱。透射电镜可见凋亡细胞的形态学改变，线粒体空泡化、核凝聚、核固缩。流式细胞术发现茶多酚可使肺癌细胞阻滞于G1期，以24小时、150mg／L阻滞作用最强。结论茶多酚可诱导人肺癌SPC-A1细胞的凋亡，并使细胞周期阻滞于G1期。

Objective To study the effects of tea polyphenols（TP）on lung cancer cell line SPC- A1 proliferation and cell cycle in vitro. Methods SPC-A1 cell line were treated with TP at 50 ～ 300mg/ L. Cell proliferation were evaluated by MTT and apoptosis by electric microscope, blockage of cell cycle by flow cytimetry in vitro. Results The proliferation of SPC-A1 were significantly inhibited by tea polyphenols and the inhibitionrate was time-dependent（ 12 ～36h） and concentration-dependent（50 ～ 150mg/L）. Its inhibitory effect decreased when the concentration exceeds 150mg/L. Typical nuclear condensation was observed by tranmissional electron microscope and SPC-A1 cells were blocked in G, phaes. The highest G1 peak was detected at the concentration of 150mg/L after 24h. Conclusion TP can inhibited the proliferation of SPC-A1 in vitro, by inducing cancer cell apoptosis and blocking cell cycle.