纳米银粒子修饰电极法测定血红蛋白

Silver Nanoparticles Modified Electrode and Its Application to the Determination of Hemoglabin

报道了一种利用纳米材料修饰电极检测血红蛋白的新方法。制作了以纳米银粒子修饰的银电极，并研究了血红蛋白在该修饰电极上的直接电化学行为。实验结果表明，血红蛋白在该修饰电极上具有良好的电流响应。在2．0×10^-7～1．0×10^-5mol／L浓度范围内，血红蛋白的氧化峰电流与其浓度呈良好线性关系；检出限为7．4×10^-8mol／L。研究了该修饰电极对血红蛋白的催化机理，利用该电极所建立的方法实现了对血红蛋白的分析测定。

A silver electrode modified by silver nanoparticles was prepared and its application to the electrochemical derermination of hemoglobin （Hb） was studied. Silver nanoparticles were prepared by reduction of AgNO3 with NaBH4. They can be efficientyl immobilized on the surface of a silver electrode. It showed that the silver nanoparticles could clearly enhance the electron transfer process between hemoglobin and the electrode compared with bulk silver. Linear sweep voltammetric measurement of Hb at the chemical modified electrode indicated that the oxidative peak currents of Hb were linear to its concentration ranging from 2.0×10^-7～1.0×10^-5mol/L with the detection limits of 7.4×10^-8mol/L. The direct electrochemistry of Hb was also studied by cyclic voltammetry. The method has been used to detect Hb in artificial mixture sample.