采用含原位聚合阴离子交换整体柱的微芯片分离氨基酸

Separation of Amino Acid by In-situ Polymerized Anion Exchange Micro-monolithic Column on Micro-fluidic Chip

采用原位聚合法,制备了以2-甲基丙烯酰氧乙基三甲基氯化铵(META)为功能单体的强碱性季铵盐离子交换型微整体柱,构建了带微整体柱的复合式微流控芯片;以氨基酸-H2O2-Lum inol化学发光体系为样品对象,根据氨基酸等电点的差异,在原位聚合微整体柱上进行分离实验。进行了苯丙氨酸-白氨酸、苯丙氨酸-组氨酸、苯丙氨酸-精氨酸3组氨基酸混合体系的分离,获得了很好的分离结果。优化并讨论了影响氨基酸分离效果的多种因素,如缓冲液pH值、洗脱液pH值和洗脱液流速等。在优化条件下,苯丙氨酸-精氨酸的分离度达到1.6,结果显示出整体柱与微流控体系相结合的可行性。

Alkali quaternary ammonium anion exchange micro-monolithic column based on monomer of 2-（methacryloyloxy） ethyl trimethylammonium chloride （META） was polymerized in-situ. The micro-monolithic column was combined with micro-fluidic chip. The amino acids-H20：luminol chemiluminescence system was taken as samples on the combinatorial micro-fluidic chip. Due to the difference of isoelectric point（ pI）of amino acids, separation experiments were performed on the micro-monolithic column. The separation of amino acid samples including phenylalanine-leuclne, phenylalanine-histidine and phenylalanine-arginine, was carried out on the set-up microchip. The results showed that complete separation of samples was realized. Several factors effected on amino acids separation, such as buffer pH, elution pH and flow rate were optimized. Under the optimized conditions, the resolution of phenylalanine-arginine was 1.6. The results also demonstrated feasibility of integrating the micro-monolithic column on micro-fluidic chip.