含硒紫球藻胞外多糖的制备及对体内外肿瘤细胞生长的影响

Pre paration of Se-PSP and effect of Se-PSP on growth of invitro and invivo tumors

采用在培养液中添加亚硒酸来实现藻对无机硒的富集和转化,制备一种含硒的紫球藻(Porphyridiumsp.)胞外多糖(Se-PSP);通过DAN荧光法测硒含量及红外光谱,对硒是否结合在多糖上进行了初步探讨;用四唑盐比色法(MTT)研究了不同浓度含硒的紫球藻胞外多糖对3种体外肿瘤细胞生长的影响。通过建立荷瘤小鼠模型,研究了含硒多糖对腹水瘤S180的作用。结果表明含硒的胞外多糖含硒量约为0.015mg/g,40,80mg/LSe-PSP对3种肿瘤细胞SMMC7721、A549、A357均有一定程度的抑制作用,尤其是对SMMC7721抑制程度最为明显。荷瘤小鼠模型实验结果表明100mg/(kg.·d)Se-PSP可以延长荷瘤小鼠的生存时间,Se-PSP有可能成为一种抗肿瘤药物。

The exopolysaccharide containing selenium （Se-PSP） ofPorphyridium sp. was isolated from the algal solution in which H2SeO3 was appended. Selenium with exopolysaccharide was investigated by means of the methods of DAN fluorescence and IR spectrum .The antitumor activities of Se-PSP and PSP in vitro and in vivo were studied with MTT and animal experiments . According to the results of DAN fluorescence and IR spectrum, its structure was similar to that of non-selenlium exopolysaccharide of Porphyridium sp. （PSP）. Se-content of Se-PSP was 0.015 mg/g±0.05 mg/g. We also studied the effects of different concentrations of Se-PSP on growth of in vitro cells and found 40 mg/L 80 mg/L Se-PSP could inhibit multiplication of 7721 and change the cells＇ shape, 100mg/ （kg · d ） Se-PSP can postphone the form of cancer and prolong the life span of mice but the same concentration PSP couldn＇t do so. Se-PSP can become a new antitumor medication.