摘要
目的:探讨细胞生长因子(GF)对生物电场(EF)诱导大鼠主动脉血管平滑肌细胞(VSMC)定向迁移的影响。方法:体外培养大鼠主动脉VSMC,在不同条件培养基中用150 mV.mm-1EF干预6h,显微成像及图象分析技术检测平滑肌细胞迁移情况。结果:在含有10%胎牛血清(FBS)的培养基中,VSMC在EF作用下向阴极迁移。在无血清的培养基中,细胞没有定向迁移。在无血清培养基中补充血小板源生长因子-BB、碱性成纤维细胞生长因子、转化生长因子-β1(PDGF-BBb、FGF、TGF-β1)时,部分恢复细胞向阴极迁移。结论:EF诱导的VSMC定向迁移依赖于血清的存在,血清中的生长因子与EF协同作用,促进细胞向阴极迁移。
Objective：To investigate the effects of growth factor（GF） on directed migration of cultrued vascular smooth muscle cells（VSMC） among electrical field Methods：Cultured VSMC were exposed to physiological electrical field 150mV/min for 6 hours in the presence of DMEM,DMEM＋10% foctal bovine serum（FBS） or DMEM＋GF,theinterval photographs of VSMC were taken and analyzed.Results：In media containing 10% FBS,these cells migrated cathodally.The cells cultured in serum-free medium showed no directed migration.All of platelet derived growth factor-BB（PDGF-BB）,basic firbroblast growth factor（BFGF） and transforming growth factor-β1（TGF-β1） promoted VSMC directed migration in serum-free medium.Conclusion：Physiological electrical field induce VSMC directed migration.Some substances in serum are necessary to VSMC directed migration.including growth factors.
出处
《西南国防医药》
CAS
2006年第1期1-4,共4页
Medical Journal of National Defending Forces in Southwest China
基金
国家自然科学基金资助项目(No.30170236)
