血管神经化组织工程化骨动物模型的建立与意义

The establishment of animal model about vascularization and neurotizalion in tissue engineered bore

目的利用组织工程的方法建立血管神经化组织工程骨的动物模型。方法在12只6个月龄新西兰大白兔髂骨处抽取骨髓,分离骨髓间充质的干细胞并体外扩增、诱导为成骨细胞,并与β-磷酸三钙(-βTCP)复合构建组织工程化骨,修复新西兰大白兔股骨15mm骨缺损,同时将血管神经束植入材料,构建血管神经化组织工程化骨。术后第4、8、12周行影像学、组织学检查,观察血管神经化情况。结果不同时间点的影像学、组织学均显示不同程度的血管神经化现象,术后12周组织工程化骨完全骨化。结论利用组织工程的方法,采用血管神经束植入的方式,在新西兰大白兔股骨上构建血管神经化组织工程化骨的动物模型是成功的。为组织工程化骨的血管神经化研究提供了一种新的方法。

Objective： Using tissue engineering methods to construct a new animal model for the investigation of vascularization and neurotization in bone tissue engineering. Methods： Collected bone marrow from twelve six-month New Zealand rabbits＇ ilia,isolated bone marrow mesenchymal stem cells and amplified in vitro. When induced into osteoblasts,cells compound with β-TCP were used to repair 15mm femur bone defects. Meanwhile, vascular bundles and nerve tracts embedded for the purpose of reconstructing vascularized and neurotized tissue engineered bone. At 4, 8 and 12 weeks postoperatively, imageology and histology examinations were made to detect the vaseularization and neurotization of new bone. Results： Imageology and histology examinations at different time points demonstrated somewhat degree of vascularization and neurotization, at twelve weeks, completely ossification of newly formed bone was observed. Conclusions： Using tissue engineering methods combined together with imbedding of vascular bundles and nerve tracts can successfully construct a new animal model of vascularized and neurotized tissueengineered femur bone. And it providing a new method for the investigation of vascularization and neurotization of bone tissue engineering.