三十烷醇对极大螺旋藻生物量及生化组成的影响

The effects of triacontanol on biomass and biochemical composition of Spirulina maxima

在Zarrouk液体培养基中加入1g／m^33液体三十烷醇（LTA），极大螺旋藻（Spirulina maxima）的生物量增加了24．6％～25．7％，即使在碳源NaHCO3减半（实验组2）的条件下生物量仍增加了4．43％～4．92％；碳水化合物含量增加了11．9%～16．0%，在实验组2条件下也增加5．7%～8．3％；叶绿素增加率为45．8％～63.3％；蛋白质总量基本不变，但水溶性蛋白的含量明显增加，从对照组的36％增长至实验组的60％左右，而水不溶性蛋白的含量则明显从30％左右减至10％左右．从螺旋藻生产基地的中试培养结果看，加入1g／m^3液体三十烷醇，生物量提高了39．76％，比在实验室条件下获得更好的增产效果，但加入固体三十烷醇（STA）反而减少12%．以上结果表明，在极大螺旋藻培养基中添加液体三十烷醇可大幅度提高生物量和生化组成的含量．

The biomass of Spirulina maxima increased 24.6%-25.7% when being added 1 g/m^3 liquid triacontanol into Zarrouk culture medium; the biomass of Spirulina maxima still increased 4.43%-4.92% even if content of NaHCO3 in the medium was decreased half（Test 2）. The content of carbohydrate increased 11.9%-16. 0% in Test 1 and 5. 7%-8. 3% in Test 2 respectively. The content of chlorophyll a increased 45. 8%-63. 3% ; the total content of protein kept invariable basically, but hydrosoluble protein was obviously improved from 36% to 60% or so, while non-hydrosoluble protein decreased from 30% to 10% or so. Moreover,39.76% rate of increase of biomass was got when adding 1 g/m^3 liquid triacontanol on a large-scale culture at Spirulina produce base, higher than on a little-scale at lab oratory, but decreased 12% when adding solid triacontanol. So a conclusion can been obtained： the Spirulina biomass and biochemical composition were greatly improved when adding liquid triacontanol into the culture medium.