脾虚泄泻患者肠道嗜酸细胞主碱基阳离子蛋白和CD3细胞表达与食物过敏的关系

Relationship between the expression of MBP and CD3 and food allergy in patients with spleen-deficient diarrhea

[目的]观察脾虚泄泻与食物过敏及肠道嗜酸细胞炎性递质:嗜酸细胞主碱基阳离子蛋白(MBP)和CD3细胞表达的关系,探讨脾虚泄泻的免疫学机制。[方法]指导298例脾虚泄泻患者做食物日志,将症状与食物明显相关者(76例,25.5%)分为饮食调整组与对照组各38例,同时对其中9例患者十二指肠黏膜嗜酸细胞炎性递质:MBP和CD3进行免疫组化检测。[结果]饮食调整组4周症状完全缓解率、明显缓解率(23.7%、65.8%)均优于对照组(2.6%、15.8%),差异有统计学意义(P<0.01)。免疫组化检测显示其十二指肠黏膜MBP和CD3阳性细胞较对照组表达增强,差异有统计学意义(P<0.01)。[结论]部分脾虚泄泻与食物过敏所致肠道免疫机制紊乱关系密切,饮食调整有利于脾虚泄泻症状的缓解。

[Objective] To observe the relationship between food allergy and the value of the expression of CD3 and major basic protein （MBP） in patient with spleen-deficient diarrhea. [Methods] Two hundred and ninety eight patients with spleen-deficient diarrhea were instructed to note food log. Seventy-six cases whose symptoms were obviously mutual with food were choosed to treat by food- remedy （n=38） and as control （n=38） respectively. The expression of CD3 and MBP in small intestine mucosa was detected by ABC immunostaining in nine cases at the same time. [Resuits] The curative rate, effective rate after 4 weeks food-remedy were 23.7 and 65.8 G, 2.6 G and 15.8 G in the twogroups respectively （P〈0.01）. The expression of CD3 positive and MBP positive cells were higher than that in control group（P〈0.01）. [Conclusion]Some of spleen-deficient diarrheas have close relationship with food allergy. There is a significant value to treat those spleen-deficient diarrheas with the method food-remedy.