摘要
采用超声波破碎法对嗜酸乳杆菌A和B产生的β-半乳糖苷酶进行提取,并以邻硝基苯-β-D-半乳糖苷(ONPG)为底物测定了β-半乳糖苷酶的活力。通过单因素试验和正交试验,选出了超声波破碎的最佳工艺条件。实验表明,嗜酸乳杆菌A在工作时间7.6min,工作/间歇为28s/20s,温度36℃时,超声波对细胞的破碎效果较好,此条件下破碎后,A株产生的β-半乳糖苷酶活力为5.963μmolONP/L·min。嗜酸乳杆菌B在工作时间6.8min,工作/间歇为20s/20s,温度37℃时效果较好,产生的β-半乳糖苷酶活力为6.683μmolONP/L·min。因此,嗜酸乳杆菌具有较高产生β-半乳糖苷酶的能力。
The extraction of the β-galactosidase produced by La-A and La-B was studied with the ultrasonic cell-break method. Meanwhile, the activity of β-galactosidase was assayed with reagent ONPG as substrate.Through the single-factor experiment and orthogonal experiment, the optimum technology was chosen. The results showed that the optimum conditions on cell-break of La-A with the ultrasonic method were： work time 7.6s,work/terral time 28s/20s, and temperature 36℃ and B was work time 6.8s, work/intermission time 20s/20s, and temperature 37℃. In these conditions, the activity of β-galactosidase produced by La-A and La-B reached 5.963 μmol ONP/L·min and 6.683μmol ONP/L · min respectively. Therefore, Lactobacillus acidophilus had higher activity of producing β-galactosidase.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2006年第1期47-50,共4页
Food Science
基金
河南省高校杰出科研人才创新工程项目(2004KYCX009)
