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水提醇沉法从葵花籽中提取绿原酸 被引量:17

Extraction of Chlorogenic Acid from Sunflower Seeds by Water Solution and Alcohol Sedimentation
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摘要 绿原酸具有抗菌、抗病毒、抗氧化等生物活性。葵花籽富含绿原酸。以水酶法提取葵花籽油脂后分离出的水浸提液为原料,采用醇沉淀法去除浸提液中的多糖,抽滤浓缩滤液后得到绿原酸。多次实验结果表明,浸提液浓缩到固形物含量在13%时,加入浸提浓缩液体积1倍量的95%的乙醇沉淀并过滤掉多糖,反复处理两次,滤液再经真空浓缩得到纯度76%左右的绿原酸。 Chlorogenic acid has biological activity such as anti-microbes, anti-virus, oxidation prevention and so on. Sunflower seeds are full of chlorogenic acid. This experience extracted chlorogenic acid out of waste aqueous solution from water-enzyme method sunflower seeds oil extraction. The experiments results showed that when concentration of solid substance of the waste aqueous solution was condensated to 13% (W/V)on vacuum, 95% alcohol(1:1,V/V) was added to the condensated solution to sedimentate polysaccharose, sedimentation was eentrifugalized, repeated above treatment, and filtrate was collected and condensated on vacuum, then chlorogenie acid crystal of 76% purity was obtained.
出处 《食品科学》 EI CAS CSCD 北大核心 2006年第1期159-161,共3页 Food Science
基金 齐齐哈尔市科学技术局2004年农业攻关项目 黑龙江省普通高等学校骨干教师创新能力资助计划项目(1055G061)
关键词 葵花籽 浸提液 绿原酸 多糖 sunflower seeds aqueous solution chlorogenic acid polysaccharose
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