高效液相色谱法测定黄酒中生物胺的含量

Biogenic Amines Contents in Rice Wine by HPLC

本文建立了测定酿造酒(黄酒)中生物胺含量的高效液相色谱法,优化的测定条件为:样品经0.4mol/L高氯酸提取后用丹磺酰氯柱前衍生,流动相为乙腈和水,采用梯度洗脱,流速为0.8ml/min,二极管阵列检测器,检测波长为254nm。该方法检测限为:尸胺、组胺和亚精胺为0.05μg/ml,酪胺为0.1μg/ml,精胺为0.25μg/ml。线性范围为2.0～40.0μg/ml(R>0.99),回收率分别为尸胺96.6%、组胺101.94%、酪胺101.90%、亚精胺98.65%、精胺107.4%。首次采用该法测定了黄酒中的生物胺,结果表明黄酒中生物胺的种类及含量因酒的品种而异,5种生物胺平均总量为114.45μg/ml,变异范围为39.27～241.07μg/ml。

The method of detecting the contents of biogenic amine in alcoholic beverages （rice wine） by HPLC was developed. The optimized condition was： samples were extracted by 0.4mol/L HClO4, and then derived using Dns-Cl. The mobile phase was a gradient elution program with a binary mixture of acetonitrile and water. The flow rate was 0.8ml/min. Diode array detector was used in the final determination with λ =254nm. The detection limits were 0.05μg/ml for cadaverine, histamine and spermidine, 0.1μg/ml for tyramine, and 0.25μg/ml for spermine. It is linear over the range of concentrations between 2.0 and 40μg/ml （R 〉 0.99）.The recovery of the method were 96.6%, 101.94%, 101.90%, 98.65%, 107.4% for cadaverine, histamine, tyramine, spermidine and spermine, respectively. By this method the kinds and contents of biogenic amine in rice wine was firstly determined. It was noticed that the biogenic amine kinds and contents varied with different rice wine. The average of total biogenic amine in rice wine was 114.45μg/ml. The variation was 39.27～241.07μg/ml.