用一氧化氮光学生物传感器研究供体药物体外释放的通路

The release of NO donor in vitro by an optical nitric oxide biosensor

目的研究一氧化氮（NO）供体药物体外释放的通路。方法用自制NO光学生物传感器通过模拟硝普钠（SNP）释放NO的3种通路所需要的体外环境，测定了不同条件的生理溶液中SNP释放的NO。结果光照和巯基化合物是引起SNP体外释放NO的主要因素。在酸化的生理液中，NO在40．0—360．0μmol·L^-1的浓度范围内。线性关系良好（r=0．9992），检测限为4．0μmol·L^-1，日内精密度RSD=2．3％，日间精密度RSD=3．4％。干扰试验发现，L—Cys和L—GSH在中性环境下对CytC膜有干扰，但在酸性条件下无干扰。与经典方法Griess比色法比较，无显著性差异。结论所用生物传感器系统为NO供体药物的体外释放研究提供了一种较好的手段。

OBJECTIVE To study releasing of NO donor in vitro. METHODS The passage way of sodium nitroprusside （SNP） releasing NO was investigated by NO optical biosensor. The detection of NO was carried out in different physiological solutions, which simulated three different passage ways. RESULTS Photolysis and thiol reducing cause SNP releasing NO. The standard curve was linear within the range of 40.0 - 360.0μmol·L^-1 of NO in acidified physiological solution（ r = 0.9992）, the limit of detection was 4.0μmol·L^-1 with RSD within a day of 2.3% ,between days RSD of 3.4%. Both L - Cys and L - GSH had influence on CytC film in neutral solution but no influence in acidified solution. NO in solution was measured by biosensor and Griess colorimetry, respectively, and no significant difference between results of the two methods was found. CONCLUSION The biosensor system provides a good method for the study on NO donor releasing NO in vitro.