温阳中药复方肝之福对肝纤维化大鼠的抗脂质过氧化作用

Antioxidation effects of warm-yang Chinese compounds Ganzhifu mixture on liver fibrosis in rats

目的观察温阳中药复方肝之福在实验性大鼠肝纤维化形成中的防治作用,并探讨其疗效机制。方法Wistar大鼠45只,随机分为对照组、肝之福组、秋水仙碱组和模型组。除对照组外,其余3组均采用sc CCl4及饮用100 ml.L-1乙醇的方法制备大鼠肝纤维化模型。在造模的同时,肝之福组ig肝之福,秋水仙碱组ig秋水仙碱,模型组则ig等量生理盐水,每日1次,共30 d。实验结束后,分别采血测定肝功能,取肝组织检测丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性,并根据炎症活动度及纤维化半定量计分系统(SSS)评分,观察预防用药组与模型组的病理组织学变化。结果与模型对照组相比,肝之福组的肝功能明显改善,MDA含量由16.31±6.03 nmol.mg-1减少到8.84±2.08 nmol.mg-1(P<0.01);而SOD活性则由86.33±17.74 U.mg-1升高至122.01±19.12 U.mg-1(P<0.01);同时,SSS评分炎症活动度及肝纤维化程度明显改善。经比较,肝之福抗肝纤维化的疗效与秋水仙碱相当。结论肝之福可保护肝脏,预防或缓解肝纤维化的形成。抗脂质过氧化是其可能的作用机制。

OBJECTIVE To observe the effects of Ganzhifu mixture on the course of experimental hepatic fibrosis and to explore the mechanism. METHODS Forty five Wistar rats were used in this study comprising four groups： normal group, Ganzhifu group, colchicine group and model group. Except normal group, the other three groups were given subcutaneous injection of CCL4 and drinking 10% alcohol so as to make the model of hepatic fibrosis. At the same time the rats of Ganzhifu group were given the herbal remedies via gastric cannel once each day, the rats of colchicine group were given colchicine by same way, the rats of model group were given normal saline. At the end of the experiment, liver function, malondialdehyde（MDA） content, superoxide disumutase（SOD） activity of liver tissue were measured, respectively. Tissue microarray sections of liver were stained with HE, Masson and Gorden- Sweet to observe pathological changes and calculate the point according to inflammation and fibrosis semiquantative scoring system（SSS） of liver tissue. RESULTS Compared with model group, liver function was impreved remarkably MDA content changed from 16.31±6.03 nmol·mg^-1 to 8.84±2.08 nmol·mg^-1（ p 〈 0.01） accompanying with a striking rise of SOD activity from 86.33±17.74 U·mg^-1 to 122.01±19.12 U·mg^-1 （ P 〈 0.01）. Pathological findings showed that the Ganzhifu group had less inflammation and lower degree of fibrosis. The effect of Ganzhifu on liver fibrosis was parallel with colchicine. CONCLUSION Ganzhifu can protect liver and prevent or alleviate the rats hepatic fibrosis. The mechanism of Ganzhifu is antioxidation possibly.