HPLC测定秦艽中的生物碱和龙胆苦苷

Determination of alkaloids and gentiopicroside in Radix Gentianae Macrophyllae by HPLC

目的建立同时测定秦艽中秦艽甲素、秦艽丙索和龙胆苦苷含量的方法。方法采用HPLC法。结果秦艽甲素在3．28-16．92μg内线性关系良好（R^2=0．9991），平均加样回收率为94．42％，RSD=2．04％；秦艽丙素在0．05～4．87μg内线性关系良好（R^2=0．9994），平均加样回收率为95．87％，RSD=1．66％；龙胆苦苷在79—390μg内线性关系良好（R^2=0．9994），平均加样回收率为92．86％，RSD=2．19％。结论该法简便、准确、重复性好，为秦艽药材的评价提供了含量测定方法。

OBJECTIVE To establish a method for the determination of gentianine, gentianal and gentiopieroside in Radix Gentianae Macrophyllae. METHODS HPLC was adopted to determine the contents in Radix Gentianae MacrophyUae. RESULTS This method had good linearity in the range of 3.28 - 16.92μg（ R^2 = 0.9991） ,and the average recovery was 94.42% with RSD of 2.04% for gentianine. It had good linearity in the range of 0.05 - 4.87μg（ R^2 = 0.9994） ,and the average recovery was 95.87% with RSD of 1.66% for gentianal. It was linear in the range of 79 - 390μg（ R^2 = 0.9994）, and the average recovery was 92.86% with RSD of 2.19 % for gentiopieroside. CONCLUSION This method is simple, accurate, and suitable for the quality control of Radix Gentianae Maerophyllae.