原位杂交组织化学方法鉴定玉米根尖内源生长素对根冠特定基因正常表达的影响

Identification the Effect of Endogenous Auxin in Maize Root Tip on Its Root Cap Specific Gene Using in Situ Hybridization

系列浓度抗生长素(降固醇酸0.1μmol/L、1.0μmol/L、10μmol/L)处理3-天龄玉米幼苗根尖(约1cm)1天,利用原位杂交组织化学方法鉴定根尖内源生长素对根冠特定基因(CloneC103)表达产生的影响。实验结果表明:正常情况下,CloneC103在4-天龄玉米幼苗初生根根尖中心根冠外周强烈表达;降固醇酸有效抑制CloneC103正常表达,随浓度增加其抑制作用加强,尤其是10μmol/L降固醇酸仅使CloneC103在初生根中心根冠最外层微弱表达。由此表明玉米根尖内源生长素影响根冠特定基因CloneC103的正常表达;CloneC103在根冠的表达位置推测该基因正常表达可能与中心根冠外周的平衡石有关。

The root tips of 3-d-old maize seedlings （about lcm） are treated by antiauxin （clofibric acid 10-7 mol/L, 10-6 mol/L, 10-5 mol/L） one day, in situ hybridization is used to identify the influence of endogenous auxin in root tip on the expression of root cap specific gene （Clone C103）. Experimental result shows that Clone C103 has a high expression in the periphery of central cap under normal condition; and its expression is inhibited efficiently by clofibric acid, especially clofibric acid 10-5 mol/L makes very weak expression of Clone C103 in the outmost of central cap. The primary result reveals that the normal expression of Clone C103 is profoundly regulated by endogenous auxin in maize root tip, and the expression of Clone C103 maybe has some relationship with statolith in the periphery of central cap.