高效液相色谱法测定丹桃颗粒剂中原儿茶醛含量

Determination of Protocatechuic Aldehyde Contained in Dantao Granules by HPLC

目的:建立丹桃颗粒剂中原几茶醛的含量测定方法,以控制该产品的质量。方法:采用高效液相色谱法,色谱柱为Altima C18柱 (250 mm×4．6 mm,5μm),柱温为35％,流动相为甲醇-0．1％磷酸溶液(30:70),流速为1．0 mL/min,检测波长为279 nm。结果:原儿茶醛进样量在0．040 48-0．404 8μg范围内与峰面积有良好的线性关系(r=0．999 9),平均加样回收率为100．12％,RSD为1．71％。结论: 该方法简便易行、准确可靠,可用于丹桃颗粒剂的质量控制。

Objective： To estahlish a HPLC method for determinations of the content of protocatechuic aldehyde contained in Dantao granules. Methods： The assay was performed on Attima C18 column（250 mm ×4.6 mm, 5 μm） by UV detector at 279 nm with methanol-0. 1% phosphoric acid （30：70） as the mobile phase at the flow rate of 1.0mL/min. Results： The amount of protocatechuic aldehyde was linear with its area over the range from 0.040 48 to 0. 404 8 μg （r =0. 999 9）. The average recovery and the relative standard deviation of protocatechuic aldehyde were 100. 12% and 1.71% （n=6）, respectively. Conclusion： The method appeared to be sensitive, accurate and can be used for the quality control of Chinese medicine Dantao granules.