紫外分光光度法测定醋酸洗必泰含漱剂中醋酸洗必泰含量

Determination of Chlorhexidine Acetate in Chlorhexidine Acetate Gargle by UV Spectrophotometer

目的:建立醋酸洗必泰含漱剂中醋酸洗必泰的含量测定方法。方法:采用紫外分光光度法,以乙醇为溶剂,测定波长为(259±1)nm。结果:醋酸洗必泰浓度在3．0-15．0 μg/mL范围内和吸收度有良好线性关系,回归方程为A=0．060 78 C-0．001 21,r=0．999 8(n=7), 平均回收率为99．78％,RSD=0．43％(n=5)。结论:该方法灵敏度高、操作简便、专属性好、重现性好,可用于该制剂的质量控制。

Objective： To develope an assay method fbr the determination of chlorhexidine acetate in chlorhexidine acetate gargle by UV spectrophotometer. Methods： UV spectrophotometer was used to determine chlorhexidine acetate. The solvent was alcohol and the wavelength was （259 ± 1） nm. Results： Chlorhexidine acetate had a good linearity between 3.0 - 15.0μg/mL, the regression equation was A = 0, 060 78 C - 0. 001 21, r = 0. 999 8 （ n = 7）, The average recovery was 99.78%, RSD = 0. 43% （ n = 5）. Conclusion： The method is simple and accurate in the quality control of chlorhexidine cetate gargle.