脾脏树突状细胞在多器官功能障碍综合征中的变化及意义

Changes in spleen dendritic cells in multiple organ dysfunction syndrome in mice

目的 探讨脾脏树突状细胞（DCs）在多器官功能障碍综合征（MODS）外周免疫器官损伤中的病理过程和作用机制。方法 复制小鼠MODS动物模型，采用光镜、电镜、免疫组化（CD205、CD86）与末端脱氧核糖转移酶介导的生物素化脱氧尿嘧啶缺刻标记技术（TUNEL）等方法观察脾脏病理学及其DCs在MODS病程中的动态变化。结果 实验早期（3h），DCs显著增生且功能活跃，伴大量淋巴细胞凋亡，外周血CD4^＋／CD8^＋比值下降；晚期（10～12d）大量DCs失活，脾脏各种免疫细胞退变、凋亡，外周血CD4^＋／CD8^＋比值显著下降。结论 脾脏DCs数量和活性的变化在MODS小鼠建立外周免疫应答和维持机体免疫平衡过程中具有重要作用。

Objective To explore the pathological features of the splenic dendritic cells （DCs） and their role in the pathogenesis of multiple organ dysfunction syndrome （MODS）. Methods The MODS model of mice was reproduced. The changes in DCs in the spleen in mice with MODS were studied using light microscope, electronic microscope, immunohistochemistry （CD205, CD86） and transferase mediated dUTP-biotin nick end labeling （TUNEL）. Results In the early stage （3 hours）, there was hyperplasia with increased activity of I）Cs in the spleen, accompanied by apoptosis of a large number of lymphocytes. CD4^＋/CD8^＋ ratio declined in the peripheral blood. In the MODS stage （10 - 12 days）,the number of DCs increased but with declined function. CD4^＋/CD8^＋ ratio declined markedly in peripheral blood. Conclusion During the course of MODS, changes in the number and activity of DCs in spleen are very important in the establishment of immune response and maintenance of immune function.