慢性肾小球肾炎伴肾衰患者血清α_1肾上腺素受体和抗血管紧张素Ⅱ受体1型自身抗体检测结果分析

Analysis of the Autoantibodies against AT1 Receptor and α1 Receptor in Chronic Glomerulonephritis with Renal Failure

目的探讨抗血管紧张素Ⅱ受体1型(AT1-受体)、α1-肾上腺素受体(α1-受体)、自身抗体是否与慢性肾小球肾炎(CGN)发病有关。方法以合成的AT1受体和α1受体多肽片段为抗原,应用酶联免疫吸附测定(ELISA)技术,检测66例CGN患者、58例高血压病患者及40例正常人血清中抗AT1和α1受体自身抗体。结果CGN肾功能不全组抗AT1和α1受体抗体阳性率分别为56.1%(37/66)和53.0%(36/66),高于高血压无肾损害组(分别为15.5%和12.1%)及正常对照组(分别为10%和12.5%),P<0.01。结论抗G蛋白偶联型受体自身抗体可能与慢性肾小球肾炎发病有关。

Objective To explore the role of the autoantibodies against angiotensin II type 1 receptor （AT1-receptor） and α1- adrenergic receptor（arreceptor）in the development of chronic glomerulonephritis （CGN） with renal failure. Methods The epitopes of the second extracellular loop of AT1 receptor （165-191）, α1 receptor （192-21）, M2 receptor （169-191） were synthesized and used respectively to screen sera autoantibodies from patients with chronic renal failure （n=66）, hypertension without renal failure （n=58） and healthy blood donors （n-40, control） by ELISA. Results In patients of chronic glomerulonephritis with renal failure, the positive rates of the autoantibodies against AT1-receptor and arreceptor were 56.1% and 53.0% respectively. The positive rates were all higher than those in patients of the hypertension without renal failure （the positive rates were 15.5% and 12.1%, respectively） and in the healthy donors （10% and 12.5%, respectively） （P〈0.01）. Conclusions The results suggested that these autoantibodies against AT1- receptor and α1-receptorr may play important roles in the pathogenesis of the CGN with renal failure patients.