环氧合酶-2在星形细胞肿瘤组织中的表达及临床意义(英文)

Expression of cyclooxygenase-2 in astrocytic tumours and its clinical significance

目的探讨COX-2的表达与星形细胞肿瘤发生、发展的关系。方法应用免疫组化技术和RT-PCR技术检测各级别星形细胞肿瘤组织及非肿瘤组织中COX-2蛋白和mRNA的表达水平。结果COX-2蛋白在各组中的表达强度(IODA值)分别为:非肿瘤组织(2.19±0.88),肿瘤组织(6.38±3.68);COX-2mRNA在各组中的表达阳性率分别为:非肿瘤组织(33.3%),肿瘤组织(83.3%);COX-2mRNA的相对含量分别为:非肿瘤组织(43.49±19.73),肿瘤组织(119.67±46.56);COX-2在肿瘤组织中的表达显著高于其在非肿瘤组织中的表达(P<0.05)。结论COX-2的过表达与星形细胞肿瘤的发生、发展关系密切,是引起肿瘤细胞恶性表型的相关基因之一;毛细胞型星形细胞瘤与弥漫性浸润性星形细胞肿瘤的遗传基础不同,是一种在生物学和形态学上所发生的基因改变不同于其它胶质瘤的星形细胞肿瘤;COX-2除参与肿瘤形成、炎症的发生等病理过程外,在人体器官发育及正常生理功能的维持过程中也起到比预期更为复杂的作用。

[Objective] To investigate the relationship between cyclooxygenase-2（COX-2） and occurrence and development of astrocytic tumours. [Methods] Immunohistochemistry technique and reverse transcription polymerase chain reaction （RT-PCR） technique were adopted to examine the levels of expression of COX-2 protein and mRNA in astrocytic tumours and non-tumour tissues. [Results] The expression of COX-2 protein in each group by immunohistochemical staining （IODA） was： non-tumour tissues（2.19±0.88）, astroeytie tumours（6.38±3.68）, pilocytic astrocytoma （3.54 ± 1.29）, diffuse astrocytoma （5.58±2.78）, anaplastie astrocytoma （6.88±2.70）, glioblastoma （9.19 ± 4.62）; the rate of positive expression of COX-2 mRNA was： non-tumour tissues （33.3%）, astrocytic tumours （83.3%）; the relative content of COX-2 mRNA was： non-tumour tissues（43.49±19.73）, astrocytie tumours（ 119.67± 46.56）; the expression of COX-2 in astrocytic tumours was significantly highter than in non-tumour tissues （P 〈 0.05）. [ Conclusions] The over-expression of COX-2 has close relation with occurrence and development of astrocytie tumours; Pilocytic astroeytoma is different from other astrocytic tumours in change of gene in biology and morphology; COX-2 has complicated action in the course of organ growth and maintaining normal physiological function besides COX-2 joins the pathological courses of tumourigenesis and inflammatory occurrence etc.