尿液中Survivin定量测定在膀胱移行细胞癌诊断及随访中的临床价值

Diagnosis and follow up value of quantitative examination of Survivin in urine in patients of BTCC

目的探讨尿液中Survivin定量测定在膀胱移行细胞癌(BTCC)诊断及随访中的临床价值。方法采用酶联免疫测定(ELISA)法定量测定43例BTCC患者尿液中Survivin含量,同时对10例BTCC术后复查阴性患者;9例癌前病变患者;14例其它泌尿系疾病患者;16例健康对照者进行检测。结果BTCC组尿液中Survivin含量显著高于其它组,Survivin含量随BTCC分期、分级增高而增加,初、复发肿瘤间Survivin含量差异无显著。截取值0.74μg/L时尿Survivin测定在诊断BTCC中敏感性为93%,特异性为83.7%。结论BTCC患者尿液中Survivin水平明显升高并且同肿瘤的侵袭状态密切相关,尿液中Survivin定量测定有可能成为一项有价值的用于BTCC筛查诊断和术后监测的新指标。

[Objective] To explore the clinical diagnoses and follow up value of quantitative examination of Survivin in the urine for bladder transitional cell carcinoma （BTCC） patients for diagnosis and investigation. [Methods] Urine samples of 43 BTCC patients were examined by the quantitative Enzyme-linked immunoadsordent assay （ELISA）. At the same time, 10 cases that underwent surgical procedure previously for BTCC but now free of the disease, 9 cases of precancerous lesions, 14 cases of other urological diseases and 16 healthy volunteers were examined as the matched control groups. [Results] The level of Survivin in BTCC group was significant higher than other groups and the contents of Survivin increased with tumor grade and stage progression. There were no significant differences between the primary tumors and recurrences. Using the cutoff value 0.74 p,g/L, the sensitivity and specificity were 93% and 83.7%, respectively. [Conclusion] The contents of Survivin in urine of BTCC patients is significanly higher than that in healthy individuals and other patients with urological diseases, urine Survivin is closely related to the invasive behavior of the tumors. Urine ELISA detection of Survivin may be a new useful means for screening diagnosis and monitoring of BTCC patients.