直流电治疗诱导宫颈癌HELA细胞凋亡与外环境离子浓度变化关系的研究

Study on relationship between cervical cancer hela cell apoptosis inducing by electrochemical therapy and changes of different ionic concentration in external environment

目的探讨电化学治疗过程中细胞凋亡发生与外环境离子浓度变化的关系。方法采用全自动生化分析仪测定电化学治疗后宫颈癌HELA细胞外环境中各种离子浓度的变化,流式细胞仪测定治疗前后细胞凋亡的发生率。结果电化学治疗(5V,10C)组,阴极处K+、Ca2+、Mg2+浓度明显高于对照组,Cl-浓度明显低于对照组;阳极处Mg2+浓度明显低于对照组,Cl-浓度明显高于对照组。(10V,10C)组阴极K+、Na+浓度明显高于对照组,Cl-浓度明显低于对照组,阳极处Na+浓度明显低于对照组,Cl-浓度明显高于对照组。(5V,5C)、(10V,10C)、(5V,10C)组凋亡率分别为18.46%、29.13%、67.77%,与对照组相比差异显著(P<0.05)。结论电化学治疗阴极处K+、Ca2+、Mg2+浓度升高,阳极处Cl-浓度明显高于对照组,离子浓度变化幅度和肿瘤细胞凋亡发生率在低电压高电量组更明显。

[Objective] To explore the relationship between apoptosis inducing by electrochemical therapy and the changes of different ionic concentration. [Method] Automatic biochemistry analyzer was used to determine the change of different ionic concentration. Flow cytometer was used to assay the percentage of apoptosis after Electrochemical Therapy. [Result] After Electrochemical Therapy the concentration of K^＋, Ca^2＋, Mg^2＋ at cathode of （5V, 10C） is significantly higher than contrast group. The concentration of C1 at cathode is significantly lower than contrast group, the concentration of Mg^2＋ at anode of （5V, 10C） is significantly lower than contrast group and the concentration of Clˉ is higher. The concentration of K^＋, Na^＋ at cathode of （10V, 10C） is significantly higher than contrast group. The concentration of Clˉ at cathode of is significantly lower than that of contrast group. The concentration of Na^＋ at anode of （10V, 10C） is significantly lower than that of contrast group and the concentration of Clˉ is higher. The apoptosis percentage＇of （5V, 5C）, （10V, 10C）, （5V, 10C） groups are 18.46%, 29.13%, 67.77% respectively. The difference between the group of electrochemical therapy and the contrast group is significant （P 〈 0.05）. [ Conclusion] The concentration of K^＋, Ca^2＋, Mg^2＋ increase at cathode while Clˉ increase at anode. The change of ionic concentration and the percentage of apoptosis are especially obvious at low voltage and high quantity of electroeity.