一个水貂近交群体的DNA指纹分析

DNA FINGERPRINTING OF AN INBRED MINK POPULATION

从一个长期闭锁繁育的水貂群体采集鲜肝样本，分离基因组ＤＮＡ，用人源小卫星探针３３．１５和内切酶ＨａｅⅢ制备ＤＮＡ指纹图谱。计算各项ＤＮＡ图谱特征指数：无亲缘关系个体的平均带纹相似系数达０．７２，平均带纹频率为０．６４。实验结果反映了抽样群体具有较高的近交程度，与其繁育方式和群体的实际情况相吻合，表明ＤＮＡ指纹技术可有效地应用于水貂群体的近交程度分析。

Fresh liver samples were collected from a long-term closed bred mink population and the genomic DNA was isolated. DNA fingerprints were prepared with human minisatellite probe 33.15 and endonuclease HaeⅢ. Characteristic indexes of the DNA fingerprints were computed:average band sharing and average band frequency of the unrelated individuals were 0.72 and 0.64, respectively.The results showed a quite high degree of inbreeding in the sampling population, which was coincident with its breeding system and the actual situation of the investigated population.These results indicate that DNA fingerprinting technique is sensitive in assessment of inbreeding level for mink populations.