不同超数排卵方法所获羔山羊卵母细胞体外成熟率及体外受精率的比较

THE EFFECTS OF SUPEROVULATION METHODS ON IN VITRO MATURATION AND IN VITRO FERTILIZATION OF KID'S OOCYTES

本研究观察了不同超排处理方法对羔山羊卵母细胞体外成熟及体外受精的影响，并将体外受精胚胎进行了移植，以便探讨利用羔山羊卵母细胞生产体外受精胚胎的技术途径。对２～４月龄羔山羊用ＦＳＨ、ＦＳＨ＋ＬＨ（静脉注射）和ＦＳＨ＋ＬＨ（肌肉注射）三种方法进行超排处理，平均分别回收１６．０、２０．３和１５．０枚卵母细胞。将这些卵母细胞进行２０～２４ｈ的成熟培养后，其成熟率分别为４５．５％，４４．４％和７６．５％。成熟卵母细胞经体外受精，体外发育培养后其２～４细胞胚胎的发生率分别为５．６％ａ、６．７％和３１．７％ｂ（ｂ，ｖｓ，ａ，Ｐ＜０．０５）。将９枚２～４细胞期胚胎移植给６只受体母羊，结果有２只妊娠并产羔２只。

The effects of different superovulation methods on in vitro maturation(IVM) and in vitro fertilization(IVF)of kid's oocytes were observed in this research ,and the embryos derived from IVF were transferred to recipients.The research purpose was to get a method that produce IVF embryos from kid's oocytes.The 2-4 months old kids were superovulated with FSH or FSH+LH(intravenous injection) or FSH+LH(intramuscular injection).The average number of superovulatory oocytes from each kid was 16.0, 20. 3 and 15.0respectively.The maturation rates of oocytes were 45.5%, 44.4%and 76.5%after being cultured for 20-24 hours in vitro,and the development rates of 2-4 cell stage embryo were 5.6%a,6.7%and 31.7%h(b, vs.a,P<0.05)after IVF and in vitro culture,respectively.Nine IVF embryos were transferred to 6 recipients, and 2 recipients became pregnant and 2 kids were obtained.