摘要
目的:探讨乙酰肝素酶的基因表达,对卵巢癌细胞转移侵袭能力的影响。方法:用Boyden小室体外侵袭实验比较两株卵巢癌细胞HO-8910和HO8910-PM的体外侵袭能力强弱;采用半定量逆转录聚合酶链反应(RT-PCR)和原位杂交检测细胞乙酰肝素酶mRNA的表达情况;用MTT观察细胞生长能力的变化。结果:MTT检测结果显示卵巢癌细胞HO8910-PM细胞生长速度较快,而HO-8910细胞生长速度较慢。RP-PCR提示及原位杂交结果提示乙酰肝素酶在高转移性HO8910-PM细胞中表达强于转移性HO-8910细胞。Boyden小室体外侵袭实验表明,卵巢癌细胞HO8910-PM体外侵袭力较强,其穿膜细胞数为20.1±0.312,而HO-8910细胞体外侵袭力较弱,其穿膜细胞数为11.3±0.024。两者差异具有显著性意义(P<0.05)。结论:乙酰肝素酶基因的表达,能促进瘤细胞的生长和转移能力。
Objection： To investigate the effects of heparanase gene on the invasive ability of ovarian cancer cells. Methods： Boyden chamber in vitro invasion assay was used to detect the invasion capacity in vitro in two ovarian tumor cell lines HO-8910 and 8910-PM. The mRNA expression level of heparanase in cell lines HO-8910 and HO8910-PM was evaluated by semi-quantitative RP-PCR and ISH（in situ hybridization）. MTT was used to observe the change of proliferation of the two ovarian tumor cell lines. Results： Boyden chamber in vitro invasion assay indicated that the mean invasion percentage of HO-8910 cells （11. 3±0. 024）was significantly lower than that of 8910-PM （20. 1±0. 312）（P〈0. 05）. Compared with HO-8910 cells, HO8910-PM ceils expressed significantly higher levels of mRNA of heparanase by RT-PCR and ISH. The growth of HO8910-PM cell was quick, but HO-8910 ceils grew slowly. Conclusion： It is suggested that human heparanase gene can facilitate the growth and metastasis of ovarian cancer cell lines HO-8910 and HO8910-PM.
出处
《陕西医学杂志》
CAS
北大核心
2006年第2期134-137,共4页
Shaanxi Medical Journal
