IgY联合乳酸杆菌防治阴道白色念珠菌感染的实验研究

A study of the effects of IgY and Lactobacillus on the prevention and treatment of vaginal infection due to Candida albicans

目的:观察特异性抗白色念珠菌IgY和乳酸杆菌的体内外生物学效应,探讨将二者复合应用的可能性。方法:分离人阴道上皮细胞,用抗白色念珠菌IgY和乳酸杆菌对阴道上皮细胞预处理后进行白色念珠菌黏附抑制试验;建立小鼠白色念珠菌性阴道炎模型,通过阴道局部给抗白色念珠菌IgY和乳酸杆菌来观察其疗效。结果:抗白色念珠菌IgY和乳酸杆菌均能抑制白色念珠菌黏附阴道上皮细胞,并能减少白色念珠菌在小鼠阴道内的定植和减轻小鼠阴道局部的炎症反应。结论:特异性抗白色念珠菌IgY具有良好的体内外生物学效应且优于单独使用乳酸杆菌,二者复合应用效果最佳。

Objective：To observe the bio-activity of anti-Candida albicans IgY and Lactobacillus in vivo and intro and to study the possibility of combined application of them. Methods ： The human vaginal epithelium cells were isolated and adherence inhibition experiment was performed after pretreating C.albicans and buccal Epithelium cells with anti-C.albicans IgY and Lactobacillus. After the mouse model of vaginitis infected with C.albicans, therapeutic effects of anti-C.albicans IgY and Lactobacillus administered vaginally were observed. Results： Anti-C.albicans IgY and Lactobacillus can inhibit adherence of Candida albicans to vaginal epithelium cells and reduce the vaginal C. albicans colonization as well as alleviate typical signs of mouse with C. albicans colonization. Conclution ：Anti-C.albicans IgY is of good biological effect against Candida albicans in vivo and intro, and is better than that of Laetobacillus used independently, suggesting that the combined use of IgY and Lactobacillus can result in ideal effect.