一氧化氮及内皮素1在急性肾功能衰竭中的作用

Significance of nitric oxide and endothelin-1 in acute renal failure

目的观察一氧化氮(NO)和内皮素1(ET-1)含量变化在急性肾功能衰竭(ARF)中的作用。方法实验用肌肉注射甘油和皮下注射HgCl2法复制成大鼠ARF模型,以硝酸还原法、化学显色法分别检测血清及肾组织NO含量及其一氧化氮合成酶NOS、i NOS活性,并用放免检测和免疫组化方法对ARF大鼠肾组织中ET-1进行定量与定位分析。结果模型组NO含量及其合酶活性均显著增加,ET-1水平在24h明显增高,以后逐渐降低。结论NO及其合酶、ET-1在ARF的发生、发展过程中均起着重要的作用。

Objective To study the role of nitric oxide （NO） and endothelin-1（ET-1） in acute renal failure （ARF） induced by ischemia-reperfusion in rats. Methods A model of acute renal failure was established by injecting glycerin muscularly and HgCl2 subcutaneously in Wistar rats. The chemical colorimetry and nitric acid reductase method were used for detecting the content of NO, NO synthase （NOS） and inducible nitric oxide synthase （iNOS） activity in plasma and renal homogenization of ARF rats. The immunohistochemical method was used in study of the distribution and content of endothelin-1 （ET-1）. At the same time, the contents of ET-1 in plasma and renal homogenization of ARF rats were determined by radioimmunoassay. Results Compared with those in control groups, the contents of ET-1, NO, NOS, iNOS activity were significantly increased at 24 h. Conclusion The changes of NO, NOS and ET-1 in renal and plasma might play an important role in the process of ARF.