应用凝血反应检测褐家鼠种群抗药性水平

Anticoagulant Resistance Monitoring with Blood Clotting Response Tests in Rattus norvegicus

目的 测定褐家鼠的抗药性水平，比较凝血反应与摄食实验测定结果的差异。方法 采用凝血反应法和摄食法分别测定褐家鼠对杀鼠灵和溴敌隆的抗药性。结果 褐家鼠对杀鼠灵ED50N3．849（雄）和4．727mg／kg（雌），对溴敌隆ED50为0．516（雄）和0．689mg／kg（雌）。按照鼠药抗性委员会（ARRPC）推荐的敏感褐家鼠基准，该褐家鼠种群对杀鼠灵的抗性水平在2．219～2．549之间，对溴敌隆的抗性水平在1．098～1．129之间。摄食实验也获得了类似结果。结论 凝血反应是一种简便、可行的褐家鼠抗药性测试方法。

Objective Evaluation the resistance of Rattus norvegicus against warfarin and bromadiolone and comparison the two testing methods, blood clotting response （BCR） and feeding. Methods The resistance of R. norvegicus against warfarin and bromadiolone was tested with BCR and feeding method, Results The 50 % effective dosees （ED50 of warfarin for R. norvigicus Beijing suburb population were 3.849 mg/kg and 4.27 mg/kg for male and female respectively, As for bromadiolone, ED50 values were 0. 516 mg/kg and 0.689 mg/kg for male and female, According to the baseline of susceptible R, norvegicus approved by ARRPC, the resistance of the population was 2,219-2. 549 fold for warfarin and 1.098-1.129 for bromadiolone, Conclusion Compared with feeding test approved by WHO in 1980s, BCR methods obtained similar results and appeared to be a reliable and applicable method in monitor anticoagulant resistance of commensal rodent,