摘要
目的研究油茶皂素不育剂对鼠类的抗生育功能。方法测定不同浓度油茶皂素不育剂对雄性小鼠的体外杀精效果和体内抗生育能力。结果油茶皂素不育剂对雄性小鼠呈现出较优的剂量依赖性体外杀精活性,3 min时的最低杀精浓度为0.05 mg/ml,与人用杀精剂壬苯醇醚相当,比扁桃酸(0.1 mg/ml)和柠檬酸(0.25 mg/ml)低。与生理盐水对照组比较,125 mg/(kg.d)油茶皂素不育剂灌胃给药4周后,可以明显降低雄性小鼠的活精子数(92.1%)和精子活力(52.6%),可以显著减少雌性小鼠的妊娠率(64.0%)和活胎数(67.4%)。结论油茶皂素不育剂具有较强的体外杀精活性和体内抗生育功能,可进一步开发成体外杀精避孕药和环境友好型鼠类抗生育药剂。
Objective To study the antifertility function of sasanqua saponin sterilant against rodents. Methods The spermicidal effect in vitro and the antifertility ability in vivo on male mice of the sasanqua saponin sterilant in different concentration were mensurated. Results The spermicidal effect in vitro on male mice of the sasanqua saponin sterilant was provided with excellent positive relation in dosage-effect. The minimum spermieidal concentration being 0.05 mg/ml in 3 min of the sasanqua saponin sterilant was corresponded with nonoxynol spermicide and was lower than mandelic acid(0.1 mg/ml) and citric acid(0.25 mg/ml). Compared with the control group, after ig 125 mg/(kg·d) sasanqua saponin stefilant for 4 weeks, 92.1% alive sperm and 52.6 % sperm motility on male mice could be obviously reduced, and 64.0 % gravidity rate and 67.4 % alive embryo on female mice could be significantly decreased. Conclusion The sasanqua saponin sterilant was provided with obvious spermicidal effect in vitro and antifertility function in vivo. The sasanqua saponin spermicide or prophylactic and the antifertility medicament against rodents with entironment amity should be exploited in future.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
北大核心
2006年第1期11-14,共4页
Chinese Journal of Vector Biology and Control
基金
福建省科技重大专项基金(2004YZ02-4)
福建省教育厅科技基金(JB04310)
福州大学SRTP(05202)资助项目
关键词
油茶皂素
不育剂
鼠类
杀精作用
抗生育功能
Sasanqua saponin
Sterilant
Rodents
Spermicidal effect
Antifertility function