摘要
目的通过研究几种生物化学指标在德国小蠊实际抗性监测中的应用,进一步探索和完善德国小蠊的抗性监测方法。方法从山东省东营、青岛、济南3市分别采集野外生活的德国小蠊个体,参照GB 13917.1、13917.8-92《农药登记卫生杀虫剂室内药效试验方法》喷施一定剂量不同浓度的高效氯氰菊酯,用各种酶测定试剂盒测定德国小蠊的酶活性。结果用乙酰胆碱酯酶(AChE)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GPx)的活性作为指标监测抗性种群的抗性状况,济南、青岛、东营3市德国小蠊的抗性指数依次为1.47、1.35、1.49,1.29、1.25、1.35和1.33、1.29、1.37,抗性种群与敏感品系的酶活性经统计学处理差异有统计学意义(P<0.05);用Na-K-ATPase、Ca-ATPase的活性作为指标监测抗性种群的抗性状况,济南、青岛、东营3市德国小蠊的抗性指数依次为1.02、1.04、1.03和1.16、1.08、1.19,与敏感品系的酶活性差异无统计学意义(P>0.05)。结论AChE、SOD、GPx的酶活性可作为指示德国小蠊对高效氯氰菊酯抗性状况的指示指标,而Na-K-ATPase或Ca-ATPase的酶活性只能作为参考指标。
Objective By studying the biochemistry indexs in BlatteUa germanica resistant monitoring, to know the resistance of B. germanica. Methods Field populations of B. germanica were captured from Jinan, Qingdao and Dongying. Beta cypermethrin at different concentration was sprayed based on the method of GB 13917. 1,13917.8-92 for insecticide registered testing indoor effect. The enzymes activities were tested using kits. Results There were significant differant activities of AChE,SOD and GPx compared with cypermethrin treated population and susceptible strain, while the activities of Na-K-ATPase and Ca-ATPase weren't significantly different between them. Conclusion The activities of ACHE, SOD and GPx may be effective indexes of resistant monitoring to beta-cypermethrin and Na-K-ATPase and Ca ATPase activities can only be reference indexes.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
北大核心
2006年第1期39-41,共3页
Chinese Journal of Vector Biology and Control
基金
山东省卫生厅科技发展计划项目(HZ0990)
关键词
德国小蠊
氯氰菊酯
抗药性
Blattella germanica
Cypermethrin
Resistance