摘要
目的研究怀牛膝(Achyranthcs bidentata Blume,BL)水煎剂对2型糖尿病大鼠脑神经生长因子(nerve growth factor,NGF)基因mRNA表达的影响。方法随机选取以不同剂量怀牛膝水煎剂灌胃的2型糖尿病大鼠每组10只,以生理盐水灌胃的2型糖尿病大鼠及正常对照组大鼠各10只,用RT-PCR法测定脑神经生长因子基因mRNA的表达。结果 2 型糖尿病大鼠脑神经生长因子基因mRNA的表达下降,服用怀牛膝水煎剂的2型糖尿病大鼠脑神经生长因子基因mRNA的表达上调。结论怀牛膝水煎剂能增加2型糖尿病大鼠脑神经生长因子基因mRNA的表述。
Objective： To study the effect of Achyranthes bidentata Blume （BL） on the expression of the brain nerve growth factor mRNA in type 2 diabetic rats. Methods： Selecting type 2 diabetic rats with taken diverse dosage Achyranthes bidentata Blume （BL） and Physiological saline each ten rats in every group and natural ten rats in random , the expression of brain NGF mRNA was mensurated by RT- PCR. Results： The mRNA expression of Brain NGF in type 2 diabetic groups were lower than that of natural group （P 〈 0. 01）, and that Achyranthes bidentata Blume （BL） groups were higher than that of type 2 diabetic model （P 〈 0. 01 ） . Conclusion ： Achyranthes bidentata Blume （BL） can increase the mRNA expression of brain NGF in type 2 diabetic rats.
出处
《中国优生与遗传杂志》
2006年第2期9-10,共2页
Chinese Journal of Birth Health & Heredity
关键词
怀牛膝
2型糖尿病大鼠
神经生长因子
基因表达
Achyranthes bidentata Blume （BL）
Type 2 diabetic rats
Nerve growth factor
Gene expression
