小鼠胚泡植入前、中、后子宫内膜nm3-M2动态表达

nm23 - M2 expression of mice endometria in blastocyst pre - implantation, implantation window and post - implantation.

目的研究抑癌基因nm23-M2在小鼠胚泡植入前、中、后子宫内膜的动态表达。方法采用RT-PCR及免疫组织化学方法分别检测小鼠妊娠第2天(植入前)、第5天(植入中)、第7天(植入后)子宫内膜nm23-M2表达。结果 RT-PcR测得胚泡植入前、中、后子宫内膜中均存在nm23-M2的表达,但在妊娠第5天植入窗口期(植入中)nm23 -M2 mRNA／β-actin mRNA的比值明显升高,与植入前和植入后相比较,具有显著差异(P<0．01)。免疫组织化学分析显示妊娠第5天子宫内膜nm23-M2表达为强阳性,而植入前和植入后子宫内膜nm23-M2表达多为弱阳性。表明胚泡植入窗口期子宫内膜nm23-M2表达上调。结论 nm23-M2可能参与了小鼠胚泡植入。

Objective： To analysize nm23 - M2 expression patterns of mice endometria in blastocyst preimplantation, implantation window and post. implantation. Methods. Level of nm23 - M2 expression of the endometria from day 2, day 5 and day 7 pregnancies were carried out by RT - PCR and immunobistochemistry. Results ： expression of nm23 - M2 mRNA were observed in endometria from pre - implantation, implantation window and post. implantation, but expression of nm23 - M2 mRNA is the most intensive comparing with those of pre- and post- implantation （P 〈 0. 01 ）, Analysis of immunohistochemistry showed that expression of nm23 -M2 protein is better positive in implantation window, and that expression of nm23 - M2 protein is thin positive in pre - and post - implantation. This result indicate that nm23 - M2 expression is increased in blastocyst implantation window. Conclusion： Nm23 - M2 may play an important role in blastocyst implantation.